Immunohistochemical
Paraffin embedded spleen tissue, tested with an antibody Dilution of 5 ug/ml.Immunoblot
1: 20ug HEK293T no transfection, 2: 20ug HEK293T 3Flag-MDA5/IFIH1Primary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:1000Gene Name: IFIH1Submitted by: Dr. Safia Deddouche, London Research Institute| Product Name | Rabbit anti-IFIH1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Hlcd, IDDM19, MDA-5, MDA5, MGC133047 |
| Immunogen | The immunogen for anti-IFIH1 antibody: synthetic peptide directed towards the middle region of human IFIH1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHorseHumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IFIH1 |
|---|---|
| Gene Synonyms | Hlcd IDDM19 MDA-5 MDA5 MGC133047 |
| Gene Full Name | Interferon induced with helicase C domain 1 |
| Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. IFIH1 is a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation.DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | Hlcd IDDM19 MDA-5 MDA5 MGC133047 |
| Molecular Weight(MW) | 117kDa |
| Sequence | 1025 amino acids |
Immunohistochemical
Paraffin embedded spleen tissue, tested with an antibody Dilution of 5 ug/ml.
Immunoblot
1: 20ug HEK293T no transfection, 2: 20ug HEK293T 3Flag-MDA5/IFIH1Primary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:1000Gene Name: IFIH1Submitted by: Dr. Safia Deddouche, London Research Institute| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
