Application
Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 2.5 μg/ml.Application
Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 5 μg/mL.Application
Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/mL.Application
Western blot analysis of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 μg/mL.| Product Name | MDA5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MDA5 Antibody: The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-κB. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.1) Akira S, Uematsu S, and Takeuchi O. Pathogen recognition and innate immunity. Cell 2006; 124:783-801. |
| Immunogen | MDA5 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MDA5. The immunogen is located within the last 50 amino acids of MDA5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| MDA5 antibody can be used for detection of MDA5 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IFIH1 |
|---|---|
| Alternative Names | MDA5 Antibody: Hlcd MDA5 MDA-5 RLR-2 IDDM19 RH116 Interferon-induced helicase C domain-containing protein 1 Clinically amyopathic dermatomyositis autoantigen 140 kDa CADM-140 autoantigen |
| Molecular Weight(MW) | Predicted: 113 kDa Observed: 115 kDa |
| Sequence Similarities | 3) Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide |
Application
Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 2.5 μg/ml.
Application
Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 5 μg/mL.
Application
Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/mL.
Application
Western blot analysis of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 μg/mL.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | MDA5 antibody can be used for detection of MDA5 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MDA5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MDA5 Antibody is supplied in PBS containing 0.02% sodium azide. |
