| Product Name | MDA5 Antibody [Hely-1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | RIG-I and MDA5 are highly conserved helicases involved in the innate immune response to virus. RIG-I is a member of the DEAD-box RNA helicases and is activated by cytoplasmic dsRNA and 5’-ppp RNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-1 recruits the CARD adaptor inducing IFN-beta (Cardif) in a CARD-CARD-dependent manner resulting in NF-kappaB and IRF3 activation. |
| Immunogen | Recombinant human MDA5 (aa 78-555). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIPWB |
| Immunoprecipitation: (1:100). Western Blot: (1:1‘000). | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IFIH1 |
|---|---|
| Alternative Names | Helicard Melanoma Differentiation-associated Gene 5 |
| Tissue Specificity | Recognizes human MDA5. |
| Application Notes | Immunoprecipitation: (1:100). Western Blot: (1:1‘000). |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for at least 1 year after receipt when stored at -20˚C. |
| Storage Buffer | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
