Immunohistochemical
Paraffin embedded mouse vessel and adipocytes (skeletal muscle) tissue, tested with an antibody Dilution of 5 ug/ml.Immunoblot
Lane 1: 50ug mouse embryonic fibroblast lysateLane 2: 50ug mouse PTRF-KO embryonic fibroblast lysateLane 3: 50ug mouse PTRF-KO embryonic fibroblast lysate+FLAG-PTRF proteinPrimary Antibody Dilution: 1:1000Secondary Antibody: Donkey anti-Rabbit-HRPSecondary Antibody Dilution: 1:10000Gene Name: PTRFSubmitted by: Libin Liu, Boston University.Western blot
0.625ug/mlELISA Titer: 1:312500Positive Control: NIH/3T3 cell lysate| Product Name | Rabbit anti-PTRF polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cavin, Cav-p60, AW546441, 2310075E07Rik |
| Immunogen | The immunogen for anti-PTRF antibody: synthetic peptide directed towards the middle region of mouse PTRF |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PTRF |
|---|---|
| Gene Synonyms | Cavin Cav-p60 AW546441 2310075E07Rik |
| Gene Full Name | Polymerase I and transcript release factor |
| Gene Summary | In mouse, pausing of elongation complexes is mediated by the transcription termination factor TTF-I bound to the 'Sal box' terminator downstream of the rDNA transcription unit. PTRF is a Pol I and transcript release factor for dissociation of paused ternary complexes. |
| Alternative Names | Cavin Cav-p60 AW546441 2310075E07Rik |
| Molecular Weight(MW) | 43kDa |
| Sequence | 392 amino acids |
Immunohistochemical
Paraffin embedded mouse vessel and adipocytes (skeletal muscle) tissue, tested with an antibody Dilution of 5 ug/ml.
Immunoblot
Lane 1: 50ug mouse embryonic fibroblast lysateLane 2: 50ug mouse PTRF-KO embryonic fibroblast lysateLane 3: 50ug mouse PTRF-KO embryonic fibroblast lysate+FLAG-PTRF proteinPrimary Antibody Dilution: 1:1000Secondary Antibody: Donkey anti-Rabbit-HRPSecondary Antibody Dilution: 1:10000Gene Name: PTRFSubmitted by: Libin Liu, Boston University.
Western blot
0.625ug/mlELISA Titer: 1:312500Positive Control: NIH/3T3 cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
