Application
Immunofluorescence of PTRF in human spleen tissue with PTRF antibody at 20 μg/ml.Application
Immunohistochemistry of PTRF in spleen tissue with PTRF antibody at 5 μg/ml.Application
Western blot analysis of PTRF in human spleen tissue lysate with PTRF antibody at (A) 0.5 and (B) 1 μg/mL| Product Name | PTRF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PTRF Antibody: CGL4, CAVIN, CAVIN1, FKSG13, cavin-1, Polymerase I and transcript release factor, Cavin-1 |
| Product description | PTRF Antibody: The polymerase I and transcript release factor (PTRF) enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts, regulating rRNA transcription by promoting the dissociation of transcription complexes and the re-initiation of polymerase I on nascent rRNA transcripts. PTRF also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins, translocating from caveolae to the cytoplasm after insulin stimulation. PTRF is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy.1) Jansa P, Mason SW, Hoffmann-Rohrer U, et al. Cloning and functional characterization of PTRF, a novel protein which induces dissociation of paused ternary transcription complexes. EMBO J. 1998; 17:2855-64. |
| Immunogen | Rabbit polyclonal PTRF antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PTRF. The immunogen is located within amino acids 270 - 320 of PTRF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PTRF antibody can be used for detection of PTRF by Western blot at 0.5 - 1 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTRF |
|---|---|
| Alternative Names | PTRF Antibody: CGL4 CAVIN CAVIN1 FKSG13 cavin-1 Polymerase I and transcript release factor Cavin-1 |
| Molecular Weight(MW) | 43 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | At least three isoforms of PTRF are known to exist; this antibody detects all three isoforms. It is predicted to not cross-react with other members of the cavin family. |
Application
Immunofluorescence of PTRF in human spleen tissue with PTRF antibody at 20 μg/ml.
Application
Immunohistochemistry of PTRF in spleen tissue with PTRF antibody at 5 μg/ml.
Application
Western blot analysis of PTRF in human spleen tissue lysate with PTRF antibody at (A) 0.5 and (B) 1 μg/mL| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | PTRF antibody can be used for detection of PTRF by Western blot at 0.5 - 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PTRF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | PTRF Antibody is supplied in PBS containing 0.02% sodium azide. |
