Application
Antibody used in WB on Mouse NIH-3T3 at 0.625 ug/ml.Application
Antibody used in IHC on Mouse at of 5 ug/ml.Application
Antibody used in WB on Mouse embryonic fibroblast at: 1:1000 (Lane 1: 50ug mouse embryonic fibroblast lysate, Lane 2: 50ug mouse PTRF-KO embryonic fibroblast lysate, Lane 3: 50ug mouse PTRF-KO embryonic fibroblast lysate+FLAG-PTRF protein).| Product Name | PTRF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PTRF, Cavin, Cav-p60, AW546441, 2310075E07Rik |
| Product description | In mouse, pausing of elongation complexes is mediated by the transcription termination factor TTF-I bound to the 'Sal box' terminator downstream of the rDNA transcription unit. PTRF is a Pol I and transcript release factor for dissociation of paused ternary complexes.1) Miyoshi, K., et al., (2001) Genomics 71 (2), 150-155. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse PTRF. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PTRF antibody can be used for detection of PTRF by ELISA at 1:312500. PTRF antibody can be used for detection of PTRF by western blot at 0.625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PTRF |
|---|---|
| Alternative Names | PTRF Cavin Cav-p60 AW546441 2310075E07Rik |
| Molecular Weight(MW) | 43 kDa |
Application
Antibody used in WB on Mouse NIH-3T3 at 0.625 ug/ml.
Application
Antibody used in IHC on Mouse at of 5 ug/ml.
Application
Antibody used in WB on Mouse embryonic fibroblast at: 1:1000 (Lane 1: 50ug mouse embryonic fibroblast lysate, Lane 2: 50ug mouse PTRF-KO embryonic fibroblast lysate, Lane 3: 50ug mouse PTRF-KO embryonic fibroblast lysate+FLAG-PTRF protein).| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | PTRF antibody can be used for detection of PTRF by ELISA at 1:312500. PTRF antibody can be used for detection of PTRF by western blot at 0.625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PTRF antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
