Western blot
Lane 1: 20ug untransfected human Saos2 cells Lane 2: 20ug siRUVBL transfected human Saos2 cells Primary Antibody Dilution : 1:1000 Secondary Antibody:Anti-rabbit-HRP Secondary Antibody Dilution: 1:3000 Color/Signal Descriptions:BAG2 Gene Name:Wenwei Hu, Xuetian Yue, Rutgers Cancer Institute of New Jersey. Submitted by:Western blot
1.25ug/mlPositive Control: Jurkat cell lysate| Product Name | Rabbit anti-BAG2 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RP3-496N17.2, BAG-2, KIAA0576, MGC149462, dJ417I1.2 |
| Immunogen | The immunogen for anti-BAG2 antibody: synthetic peptide directed towards the C terminal of human BAG2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BAG2 |
|---|---|
| Gene Synonyms | RP3-496N17.2 BAG-2 KIAA0576 MGC149462 dJ417I1.2 |
| Gene Full Name | BCL2-associated athanogene 2 |
| Gene Summary | BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. |
| Alternative Names | RP3-496N17.2 BAG-2 KIAA0576 MGC149462 dJ417I1.2 |
| Molecular Weight(MW) | 23kDa |
| Sequence | 211 amino acids |
Western blot
Lane 1: 20ug untransfected human Saos2 cells Lane 2: 20ug siRUVBL transfected human Saos2 cells Primary Antibody Dilution : 1:1000 Secondary Antibody:Anti-rabbit-HRP Secondary Antibody Dilution: 1:3000 Color/Signal Descriptions:BAG2 Gene Name:Wenwei Hu, Xuetian Yue, Rutgers Cancer Institute of New Jersey. Submitted by:
Western blot
1.25ug/mlPositive Control: Jurkat cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
