Application
Antibody used in WB on siRUVBL transfected human Saos2 at 1:1000 (Lane 1: 20ug untransfected human Saos2 cells Lane 2: 20ug siRUVBL transfected human Saos2 cells ).Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.Application
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.| Product Name | BAG2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.1) Arndt, V., (2005) Mol. Biol. Cell 16 (12), 5891-5900. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BAG2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| BAG2 antibody can be used for detection of BAG2 by ELISA at 1:1562500. BAG2 antibody can be used for detection of BAG2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BAG2 |
|---|---|
| Alternative Names | BAG2 RP3-496N17.2 BAG-2 KIAA0576 MGC149462 dJ417I1.2 |
| Molecular Weight(MW) | 23 kDa |
Application
Antibody used in WB on siRUVBL transfected human Saos2 at 1:1000 (Lane 1: 20ug untransfected human Saos2 cells Lane 2: 20ug siRUVBL transfected human Saos2 cells ).
Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.
Application
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | BAG2 antibody can be used for detection of BAG2 by ELISA at 1:1562500. BAG2 antibody can be used for detection of BAG2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store BAG2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
