Application
BAG2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human ovarian carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Product Name | BAG2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq].1) Arndt, V., et al. Mol. Biol. Cell 16(12):5891-5900(2005) |
| Immunogen | This BAG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-176 amino acids from the C-terminal region of human BAG2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BAG2 |
|---|---|
| Alternative Names | BAG family molecular chaperone regulator 2 BAG-2 Bcl-2-associated athanogene 2 BAG2 |
| Molecular Weight(MW) | 24 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
BAG2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human ovarian carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
