Western blot
Titration: 1.0 ug/mlPositive Control: 293T Whole CellWestern blot
Positive Control: Lane1: hFEN1 (1-336), Lane2: uninduced BL21, Lane3: 2h induced BL21, Lane4: overnight induced BL21 Primary Antibody Dilution : 1:2000Secondary Antibody : Goat anti-rabbit-HRPSecondry Antibody Dilution : 1:10,000Submitted by: Prof. Jon R Sayers, University of Sheffield Medical School| Product Name | FEN1 antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Species Reactivity | BovineHorseHumanMouseRabbitRatSheepZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FEN1 |
|---|---|
| Gene Synonyms | FEN-1 MF1 RAD2 |
| Gene Full Name | Flap structure-specific endonuclease 1 |
| Gene Summary | FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. |
| Alternative Names | FEN-1 MF1 RAD2 |
| Molecular Weight(MW) | 42kDa |
| Sequence | 380 amino acids |
Western blot
Titration: 1.0 ug/mlPositive Control: 293T Whole Cell
Western blot
Positive Control: Lane1: hFEN1 (1-336), Lane2: uninduced BL21, Lane3: 2h induced BL21, Lane4: overnight induced BL21 Primary Antibody Dilution : 1:2000Secondary Antibody : Goat anti-rabbit-HRPSecondry Antibody Dilution : 1:10,000Submitted by: Prof. Jon R Sayers, University of Sheffield Medical School| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
