Application
Western blot analysis of FEN1 using rabbit polyclonal FEN1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FEN1 gene.Application
Western blot analysis of anti-FEN1 Antibody in HepG2 cell line lysates (35ug/lane)Application
Immunofluorescent analysis of U251 cells, using FEN1 Antibody . Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).| Product Name | FEN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. This protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.1) Hiraoka L.R., Harrington J.J., Gerhard D.S., Genomics 25:220-225(1995) |
| Immunogen | This FEN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-272 amino acids from the Central region of human FEN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For IF starting dilution is: 1:100 For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | FEN1 |
|---|---|
| Alternative Names | Flap endonuclease 1 {ECO:0000255|HAMAP-Rule:MF_03140} FEN-1 {ECO:0000255|HAMAP-Rule:MF_03140} 31-- {ECO:0000255|HAMAP-Rule:MF_03140} DNase IV Flap structure-specific endonuclease 1 {ECO:0000255|HAMAP-Rule:MF_03140} Maturation factor 1 MF1 hFEN-1 FE |
| Molecular Weight(MW) | 43 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of FEN1 using rabbit polyclonal FEN1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FEN1 gene.
Application
Western blot analysis of anti-FEN1 Antibody in HepG2 cell line lysates (35ug/lane)
Application
Immunofluorescent analysis of U251 cells, using FEN1 Antibody . Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | For IF starting dilution is: 1:100 For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
