Western blot
Host: Rabbit Target Name: KPNA2 Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0ug/mlWestern blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Transfected 293TWestern blot
Host: Rabbit Target Name: KPNA2 Sample Tissue: 293T Antibody Dilution: 1.0ug/ml| Product Name | Rabbit anti-KPNA2 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IPOA1, QIP2, RCH1, SRP1alpha |
| Immunogen | The immunogen for anti-KPNA2 antibody: synthetic peptide directed towards the middle region of human KPNA2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KPNA2 |
|---|---|
| Gene Synonyms | IPOA1 QIP2 RCH1 SRP1alpha |
| Gene Full Name | Karyopherin alpha 2 (RAG cohort 1, importin alpha 1) |
| Gene Summary | Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination.The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | IPOA1 QIP2 RCH1 SRP1alpha |
| Molecular Weight(MW) | 58kDa |
| Sequence | 529 amino acids |
Western blot
Host: Rabbit Target Name: KPNA2 Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0ug/ml
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Transfected 293T
Western blot
Host: Rabbit Target Name: KPNA2 Sample Tissue: 293T Antibody Dilution: 1.0ug/ml| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
