Western blot
Primary dilution: 1ug/mL Secondary dilution: 1:2000Tested with Hut-78, K562, Raj, U937, and Indomethacin Treated Jurkat cellsImmunoblot
1. 20 ug Jurkat cell lysate2. 20 ug Hut-78 cell lysate3. 20 ug K562 cell lysate4. 20 ug Raj cell lysate5. 20 ug U937 cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:2000Gene Name: CAPN1Submitted by: Ewelina Swiderek, Institute of Immunology and Experimental Therapy, Wroclaw, PolandWestern blot
CAPN1 antibody - middle region validated by WB using HT1080 cell lysate at 1ug/mL.| Product Name | Rabbit anti-CAPN1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CANP, CANP1, CANPL1, muCANP, muCL |
| Immunogen | The immunogen for anti-CAPN1 antibody: synthetic peptide directed towards the middle region of human CAPN1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineGoatHorseHumanMousePigRabbitRatSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CAPN1 |
|---|---|
| Gene Synonyms | CANP CANP1 CANPL1 muCANP muCL |
| Gene Full Name | Calpain 1, (mu/I) large subunit |
| Gene Summary | The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. |
| Alternative Names | CANP CANP1 CANPL1 muCANP muCL |
| Molecular Weight(MW) | 82kDa |
| Sequence | 714 amino acids |
Western blot
Primary dilution: 1ug/mL Secondary dilution: 1:2000Tested with Hut-78, K562, Raj, U937, and Indomethacin Treated Jurkat cells
Immunoblot
1. 20 ug Jurkat cell lysate2. 20 ug Hut-78 cell lysate3. 20 ug K562 cell lysate4. 20 ug Raj cell lysate5. 20 ug U937 cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:2000Gene Name: CAPN1Submitted by: Ewelina Swiderek, Institute of Immunology and Experimental Therapy, Wroclaw, Poland
Western blot
CAPN1 antibody - middle region validated by WB using HT1080 cell lysate at 1ug/mL.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
