WB
Western blot analysis of Calpain 1/CAPN1 using anti-Calpain 1/CAPN1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Jurkat whole cell lysates, Lane 2: Hela whole cell lysates, Lane 3: K562 whole cell lysates, Lane 4: PC-3 whole cell lysates, Lane 5: T47D whole cell lysates, Lane 6: A549 whole cell lysates, Lane 7: rat testis tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Calpain 1/CAPN1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting SubstrateIHC
IHC analysis of Calpain 1/CAPN1 using anti-Calpain 1/CAPN1 antibody. Calpain 1/CAPN1 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Calpain 1/CAPN1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of Calpain 1/CAPN1 using anti-Calpain 1/CAPN1 antibody. Calpain 1/CAPN1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-Calpain 1/CAPN1 Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow cytometry analysis of PC-3 cell(1:100) Fluoro 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG Fluoro 488. Unlabelled sample (Red line).| Product Name | Anti-Calpain 1/CAPN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human Calpain 1 recombinant protein (Position: Q396-A555). Human Calpain 1 shares 86% amino acid (aa) sequence identity with both mouse and rat Calpain 1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CAPN1 |
|---|---|
| Gene Synonyms | CANP muCL CANP1 SPG76 CANPL1 muCANP |
| Gene Full Name | calpain 1 |
| Gene Summary | The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] |
| Alternative Names | Calpain 1 Calpain 1 catalytic subunit Calpain 1 large subunit Calpain mu type CANP CANP 1 CANP1 CANPL1 CAPN1 Micromolar calpain muCANP muCL |
| Molecular Weight(MW) | 82kDa(Observed MW 82kDa) |
| Cellular Localization | Cell membrane |
WB
Western blot analysis of Calpain 1/CAPN1 using anti-Calpain 1/CAPN1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Jurkat whole cell lysates, Lane 2: Hela whole cell lysates, Lane 3: K562 whole cell lysates, Lane 4: PC-3 whole cell lysates, Lane 5: T47D whole cell lysates, Lane 6: A549 whole cell lysates, Lane 7: rat testis tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Calpain 1/CAPN1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate
IHC
IHC analysis of Calpain 1/CAPN1 using anti-Calpain 1/CAPN1 antibody. Calpain 1/CAPN1 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Calpain 1/CAPN1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of Calpain 1/CAPN1 using anti-Calpain 1/CAPN1 antibody. Calpain 1/CAPN1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-Calpain 1/CAPN1 Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow cytometry analysis of PC-3 cell(1:100) Fluoro 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG Fluoro 488. Unlabelled sample (Red line).| Application Notes | WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
