Application
Western blot analysis of Calpain I expression in lysate from Jurkat cells treated with (Lane 2) or without (Lane 1) campothecin.| Product Name | Calpain 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Calpain 1Calpains are cytosolic, calcium-dependent, cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Type 1 Calpain requires micromolar amounts of calcium, while Type 2 Calpain requires millimolar amounts of calcium. |
| Immunogen | Available on request |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 1.0 μg/ml. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMousePigRatRabbitBovine |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CANPL1 114220 Calpain 1 P07384 CAPN1 CANP muCL muCANP 823 Calpain1 |
|---|---|
| Target | Calpain 1 |
| Tissue Specificity | This antibody detects the large subunit (80 kDa) of human Calpain 1. It does not detect the smaller subunit of human Calpain 1. |
| Cellular Localization | Cytoplasmic |
Application
Western blot analysis of Calpain I expression in lysate from Jurkat cells treated with (Lane 2) or without (Lane 1) campothecin.| Application Notes | WB: Use at a concentration of 1.0 μg/ml. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 1 mg/ml BSA, 1.5 mM sodium azide and 50% glycerol |
