| Product Name | Calpain 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [15C10] to Calpain 1Mu Calpain, also known as Calpain I, and m calpain, also known as Calpain II, are intracellular, calcium-dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. The calpains have papain-like activity, thus the -pain nomenclature. Both m calpain and mu calpain are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. |
| Immunogen | Full length human protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 15C10 |
| Host Species | Mouse |
| Tested Applications | ELISAIPWB |
| For ELISA: Use at a concentration of 0.5-1 μg/ml. IP: Use at a concentration of 1-2 μg/ml. For WB: Use at a concentration of 0.5-1 μg/ml. Detects a band of approximately 80 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRatBovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | CANPL1 114220 Calpain 1 P07384 CAPN1 CANP muCL muCANP 823 Calpain1 |
|---|---|
| Target | Calpain 1 |
| Tissue Specificity | Recognizes the 80 kD subunit of µ-calpain as well as two smaller proteins that are presumed to be autolysis products. |
| Cellular Localization | Cytoplasmic/Cell membrane |
| Application Notes | For ELISA: Use at a concentration of 0.5-1 μg/ml. IP: Use at a concentration of 1-2 μg/ml. For WB: Use at a concentration of 0.5-1 μg/ml. Detects a band of approximately 80 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 50% Glycerol, 0.15M Sodium chloride, 0.02M Sodium phosphate. pH 7.5 containing 3mM Sodium azide |
