Western blot
equine cartilage explantsImmunoblot
1. 40 ug human HK2 cell (kidney proximal tubular cell line) lysate2. 40 ug H2O2 treated human HK2 Cell lysate3. 40 ug H2O2 treated human HK2 Cell lysate4. 40 ug H2O2 treated human HK2 Cell lysate5. 40 ug H2O2 treated human HK2 Cell lysate6. 40 ug H2O2 treated human HK2 Cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Goat anti-Rabbit HRPSecondary Antibody Dilution: 1:2000Gene Name: CLUSubmitted by: The University of Queensland, School of Medicine, Centre for Kidney Disease ResearchWestern blot
CLU antibody - C-terminal region validated by WB using Fetal Brain Lysate at 1ug/ml.| Product Name | Rabbit anti-CLU polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AAG4, APOJ, CLI, KUB1, MGC24903, SGP-2, SGP2, SP-40, APO-J, NA1/NA2 |
| Immunogen | The immunogen for anti-CLU antibody: synthetic peptide directed towards the C terminal of human CLU |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HorseHumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CLU |
|---|---|
| Gene Synonyms | AAG4 APOJ CLI KUB1 MGC24903 SGP-2 SGP2 SP-40 TRPM-2 TRPM2 APO-J NA1/NA2 |
| Gene Full Name | Clusterin |
| Gene Summary | The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants. |
| Alternative Names | AAG4 APOJ CLI KUB1 MGC24903 SGP-2 SGP2 SP-40 APO-J NA1/NA2 |
| Molecular Weight(MW) | 58kDa |
| Sequence | 501 amino acids |
Western blot
equine cartilage explants
Immunoblot
1. 40 ug human HK2 cell (kidney proximal tubular cell line) lysate2. 40 ug H2O2 treated human HK2 Cell lysate3. 40 ug H2O2 treated human HK2 Cell lysate4. 40 ug H2O2 treated human HK2 Cell lysate5. 40 ug H2O2 treated human HK2 Cell lysate6. 40 ug H2O2 treated human HK2 Cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Goat anti-Rabbit HRPSecondary Antibody Dilution: 1:2000Gene Name: CLUSubmitted by: The University of Queensland, School of Medicine, Centre for Kidney Disease Research
Western blot
CLU antibody - C-terminal region validated by WB using Fetal Brain Lysate at 1ug/ml.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
