WB
Western blot analysis of Clusterin on different lysates with Rabbit anti-Clusterin antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane), Lane 2: SK-Br-3 cell lysate (20 µg/Lane), Lane 3: A431 cell lysate (20 µg/Lane), Lane 4: PANC-1 cell lysate (20 µg/Lane), Lane 5: MCF7 cell lysate (20 µg/Lane), Lane 6: T-47D cell lysate (20 µg/Lane), Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Clusterin antibody at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-Clusterin antibody at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Clusterin antibody at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Clusterin antibody at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-P
Species: Human, Site: pancreas, Sample: Paraffin-embedded section, Antibody concentration: 1/2,000.| Product Name | Clusterin Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human Clusterin aa 400-449 / 449. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:2000 IHC:1:8000 IF-P:1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CLU |
|---|---|
| Gene Synonyms | CLI AAG4 APOJ CLU1 CLU2 KUB1 SGP2 APO-J SGP-2 SP-40 TRPM2 TRPM-2 NA1/NA2 |
| Gene Full Name | clusterin |
| Gene Summary | The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq, May 2011] |
| Alternative Names | 40 antibody AAG 4 antibody AAG4 antibody Aging associated protein 4 antibody Aging-associated gene 4 protein antibody AI893575 antibody APO J antibody Apo-J antibody APOJ antibody ApoJalpha antibody 40 antibody AAG 4 antibody AAG4 antibody Aging associated protein 4 antibody Aging-associated gene 4 protein antibody AI893575 antibody APO J antibody Apo-J antibody APOJ antibody ApoJalpha antibody ApoJbeta antibody Apolipoprotein J antibody ApolipoproteinJ antibody CLI antibody CLU antibody CLU1 antibody CLU2 antibody CLUS_HUMAN antibody Clusterin alpha chain antibody Clusterin antibody Clusterin beta chain antibody Complement associated protein SP 40 40 antibody Complement associated protein SP 40 antibody Complement associated protein SP40 antibody Complement cytolysis inhibitor a chain antibody Complement cytolysis inhibitor antibody Complement cytolysis inhibitor b chain antibody Complement lysis inhibitor antibody Complement-associated protein SP-40 antibody D14Ucla3 antibody Dimeric acid glycoprotein antibody Glycoprotein 80 antibody Glycoprotein III antibody GP80 antibody Ku70-binding protein 1 antibody KUB 1 antibody KUB1 antibody MGC24903 antibody NA1/NA2 antibody RATTRPM2B antibody SGP 2 antibody SGP2 antibody SP 40 antibody SP40 antibody Sugp-2 antibody Sulfated glycoprotein 2 antibody Testosterone repressed prostate message 2 antibody Testosterone-repressed prostate message 2 antibody TRPM 2 antibody TRPM-2 antibody TRPM2 antibody TRPM2B antibody Trpmb antibody |
| Molecular Weight(MW) | 52kDa(Observed band size: 35-80kDa) |
| Cellular Localization | Nucleus, Cytoplasm, Mitochondrion membrane, cytosol, Microsome, Endoplasmic reticulum, Mitochondrion, perinuclear region, Cytoplasmic vesicle, secretory vesicle, chromaffin granule. |
WB
Western blot analysis of Clusterin on different lysates with Rabbit anti-Clusterin antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane), Lane 2: SK-Br-3 cell lysate (20 µg/Lane), Lane 3: A431 cell lysate (20 µg/Lane), Lane 4: PANC-1 cell lysate (20 µg/Lane), Lane 5: MCF7 cell lysate (20 µg/Lane), Lane 6: T-47D cell lysate (20 µg/Lane), Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Clusterin antibody at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-Clusterin antibody at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Clusterin antibody at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Clusterin antibody at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Species: Human, Site: pancreas, Sample: Paraffin-embedded section, Antibody concentration: 1/2,000.| Application Notes | WB:1:2000 IHC:1:8000 IF-P:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
