1) Large-scale serial analysis of gene expression reveals genes differentially expressed in ovarian cancer: C. D. Hough, et al.; Cancer Res. 60, 6281 (2000).
Immunogen
Clusterin (monoclonal CLI-9) antibody was raised against human clusterin (TRPM-2).
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
ICCIHCIPWB
Clusterin antibody can be used for detection of human clusterin by immunocytochemistry, immunohistochemistry (frozen sections), immunoprecipitation, and western blot (1:2,000). Method: : Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2,000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.:
Clusterin antibody can be used for detection of human clusterin by immunocytochemistry, immunohistochemistry (frozen sections), immunoprecipitation, and western blot (1:2,000). Method: : Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2,000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.:
Additional Information
Form
Liquid
Storage Instructions
Clusterin antibody can be stored at 4˚C, stable for one year.