Western blot
Positive Control: Lane 1: 50ug 293T lysate Lane 2: 50ug hCUL4B transfected 293T lysate Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:10,000 Submitted by: Mei YangWestern blot
Titration: 1.0 ug/mlPositive Control: Mouse Heart| Product Name | Rabbit anti-Cul4b polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2700050M05Rik, AA409770, KIAA0695, mKIAA0695, CUL-4B |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Cul4b |
|---|---|
| Gene Synonyms | 2700050M05Rik AA409770 KIAA0695 mKIAA0695 CUL-4B |
| Gene Full Name | Cullin 4B |
| Gene Summary | Cul4b is a core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. |
| Alternative Names | 2700050M05Rik AA409770 KIAA0695 mKIAA0695 CUL-4B |
| Molecular Weight(MW) | 111kDa |
| Sequence | 970 amino acids |
| Entrez Gene | 72584 |
|---|---|
| SwissProt ID | A2A432 |
| Protein Accession | NP_001103612 |
Western blot
Positive Control: Lane 1: 50ug 293T lysate Lane 2: 50ug hCUL4B transfected 293T lysate Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:10,000 Submitted by: Mei Yang
Western blot
Titration: 1.0 ug/mlPositive Control: Mouse Heart| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
