Application
Western blot analysis of DIAPH3 expression in non-transfected CHO (A) and mouse DIAPH3 transfected CHO (B) whole cell lysates.| Product Name | DIAPH3 Antibody (H-42) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AN,AUNA1,Dia2,diap3,DIAPH3,Diaphanous homolog 3,DRF3,mDia2 |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | DIAPH3 (diaphanous homolog 3), also known as DIAP3 or DRF3, is a 1,193 amino acid member of the formin homology protein family and is required for the correct function of various cellular processes. DIAPH3 binds to both Profilin, a protein involved in cell maintenance, and to the GTP-bound form of Rho (Rho-GTP). Binding to both of these proteins allows DIAPH3 to recruit Profilin to the membrane, in a Rho-dependent manner. At the membrane, DIAPH3 promotes Actin polymerization and is required for stress fiber formation, cytokinesis and transcriptional activation of the serum response factor (SRF). DIAPH3 also regulates Actin dynamics by coupling Src tyrosine kinase (c-Src) and Rho during Actin signaling events. DIAPH3 contains one diaph-anous autoregulatory domain (DAD) and one Rho GTPase-binding domain (GBD). When DAD and GBD are intramolecularly bound, the GBD is occupied and DIAPH3 is inactive. Interruption of the DAD-GBD bond allows the GBD to bind to Rho-GTP, thus activating DIAPH3. Seven isoforms of DIAPH3 exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIPWB |
| WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | canine,bovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DIAPH3 |
|---|---|
| Alternative Names | AN AUNA1 Dia2 diap3 DIAPH3 Diaphanous homolog 3 DRF3 mDia2 |
| Molecular Weight(MW) | 137 kDa |
| Tissue Specificity | Increased expression in S phase and mitotic cells; levels decrease as cells enter in G0/G1 phase due to proteasomal degradation (at protein level) |
Application
Western blot analysis of DIAPH3 expression in non-transfected CHO (A) and mouse DIAPH3 transfected CHO (B) whole cell lysates.| Application Notes | WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
