WB
Western blot analysis of DIAPH3 expression in the total cell lysates of 293T cells that were either not transfected (A) or transfected with DIAPH3 (B).WB
Western blot analysis of human recombinant DIAPH3 fusion protein.ICC/IF
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.| Product Name | Mouse Monoclonal antibody to DIAPH3(4D5) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against amino acids 632-729 of DIAPH3 of human origin |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB:1:100-1:1000 ICC/IF:1:50-1:500 |
|
| Species Reactivity | Human |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DIAPH3 |
|---|---|
| Gene Synonyms | AN DIA2 DRF3 AUNA1 NSDAN diap3 mDia2 |
| Gene Full Name | diaphanous related formin 3 |
| Gene Summary | This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] |
| Molecular Weight(MW) | 137 kDa |
WB
Western blot analysis of DIAPH3 expression in the total cell lysates of 293T cells that were either not transfected (A) or transfected with DIAPH3 (B).
WB
Western blot analysis of human recombinant DIAPH3 fusion protein.
ICC/IF
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.| Application Notes | WB:1:100-1:1000 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 100 µg IgG2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
