Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Product Name | DIAPH3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein diaphanous homolog 3, Diaphanous-related formin-3, DRF3, MDia2, DIAPH3, DIAP3 |
| Product description | DIAPH3 binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity).1) DeWard, A.D., et al. J. Biol. Chem. 284(30):20061-20069(2009) |
| Immunogen | This DIAPH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1061-1090 amino acids from the C-terminal region of human DIAPH3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DIAPH3 |
|---|---|
| Alternative Names | Protein diaphanous homolog 3 Diaphanous-related formin-3 DRF3 MDia2 DIAPH3 DIAP3 |
| Molecular Weight(MW) | 137 kDa |
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
