WB
WB result of DIAPH3 antibody (1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HeLa cells.WB
HeLa cells were subjected to SDS PAGE followed by western blotwith DIAPH3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.WB
WB result of DIAPH3 antibody ( 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HEK-293 cells.IHC
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using DIAPH3 Antibody at dilution of 1:50.IHC
Immunohistochemical analysis of paraffinembedded mouse testistissue slide using DIAPH3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH 9.0).IHC
Immunohistochemical analysis of paraffinembedded rat testistissue slide using DIAPH3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with TrisEDTA buffer(pH 9.0)ICC/IF
Immunofluorescent analysis of(-20°C Ethanol) fixed HeLa cells using DIAPH3 antibody at dilution of 1:400 and 488- Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | DIAPH3 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human DIAPH3 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:500 ICC/IF:1:200-1:800 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | DIAPH3 |
|---|---|
| Gene Synonyms | AN DIA2 DRF3 AUNA1 NSDAN diap3 mDia2 |
| Gene Full Name | diaphanous related formin 3 |
| Gene Summary | This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] |
| Alternative Names | DIAP3 Diaphanous-related formin-3 DRF3 MDia2 |
| Molecular Weight(MW) | 137 kDa |
WB
WB result of DIAPH3 antibody (1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HeLa cells.
WB
HeLa cells were subjected to SDS PAGE followed by western blotwith DIAPH3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB
WB result of DIAPH3 antibody ( 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HEK-293 cells.
IHC
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using DIAPH3 Antibody at dilution of 1:50.
IHC
Immunohistochemical analysis of paraffinembedded mouse testistissue slide using DIAPH3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH 9.0).
IHC
Immunohistochemical analysis of paraffinembedded rat testistissue slide using DIAPH3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with TrisEDTA buffer(pH 9.0)
ICC/IF
Immunofluorescent analysis of(-20°C Ethanol) fixed HeLa cells using DIAPH3 antibody at dilution of 1:400 and 488- Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:500 ICC/IF:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
