Application
Western blot analysis of MAPE antibody (C-term) in K562 cell line lysates (35ug/lane). MAPE (arrow) was detected using the purified Pab.Application
MAPE Antibody (C-term) (RB18763) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAPE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
MAPE Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MAPE Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRAME; MAPE; OIP4; Melanoma antigen preferentially expressed in tumors; Opa-interacting protein 4; Preferentially expressed antigen of melanoma |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MAPE OIP4 |
|---|---|
| Alternative Names | PRAME MAPE OIP4 Melanoma antigen preferentially expressed in tumors Opa-interacting protein 4 Preferentially expressed antigen of melanoma |
| Molecular Weight(MW) | 57890 Da |
| Function | Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis |
| Tissue Specificity | Expressed in testis. Detected in samples of kidney, brain and skin |
| Cellular Localization | Nucleus (Probable). Cell membrane. |
| Entrez Gene | 23532 |
|---|
Application
Western blot analysis of MAPE antibody (C-term) in K562 cell line lysates (35ug/lane). MAPE (arrow) was detected using the purified Pab.
Application
MAPE Antibody (C-term) (RB18763) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAPE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
MAPE Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
