Application
A. Western blot analysis on HeLa cell lysate using anti-MCM7 RabMAb (Cat. #AJ1465b), dilution 1:10,000.Application
B. Immunohistochemical analysi of paraffin-embedded human lung tissue using anti-MCM7 RabMAb (Cat. #AJ1465b).| Product Name | MCM7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MCM7, CDC47, MCM2, DNA replication licensing factor MCM7, CDC47 homolog;P1.1-MCM3 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1974Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:2000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDC47 MCM2 |
|---|---|
| Alternative Names | MCM7 CDC47 MCM2 DNA replication licensing factor MCM7 CDC47 homolog P1.1-MCM3 |
| Molecular Weight(MW) | 81308 Da |
| Function | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage |
| Tissue Specificity | A synthetic peptide corresponding to residues near the C-terminus of human MCM7 was used as an immunogen. |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 4176 |
|---|
Application
A. Western blot analysis on HeLa cell lysate using anti-MCM7 RabMAb (Cat. #AJ1465b), dilution 1:10,000.
Application
B. Immunohistochemical analysi of paraffin-embedded human lung tissue using anti-MCM7 RabMAb (Cat. #AJ1465b).| Application Notes | WB:1:2000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
