Application
Figure 1: Western blot analysis using anti-MDM4 mAb against Hela (1), A549 (2) and A431 (3) cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human cerebra (left) and lung carcinoma (right) tissues, showing nuclear localization with DAB staining using anti-MDM4 mAb.Application
Figure 3: Confocal immunofluorescence analysis of Hela (left) and L-02 (right) cells using anti-MDM4 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.| Product Name | MDM4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HDMX; MDMX; MRP1; MDM4 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2D10F4 |
| Host Species | Mouse |
| Tested Applications | WBIHCIF |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MDMX |
|---|---|
| Alternative Names | HDMX MDMX MRP1 MDM4 |
| Molecular Weight(MW) | 54864 Da |
| Function | Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions |
| Tissue Specificity | Expressed in all tissues tested with high levels in thymus |
| Cellular Localization | Nucleus. |
| Entrez Gene | 4194 |
|---|---|
| Nucleotide Accession | NP_002384.2 |
Application
Figure 1: Western blot analysis using anti-MDM4 mAb against Hela (1), A549 (2) and A431 (3) cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human cerebra (left) and lung carcinoma (right) tissues, showing nuclear localization with DAB staining using anti-MDM4 mAb.
Application
Figure 3: Confocal immunofluorescence analysis of Hela (left) and L-02 (right) cells using anti-MDM4 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human MDM4 expressed in E. Coli. |
