Application
Figure 1: Western blot analysis using anti-MLH1 mAb against Hela (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS (6) cell lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human rectum cancer (left) and ovarian cancer (right) tissues, showing nuclear localization with DAB staining using anti-MLH1 mAb.Application
Figure 3: Confocal immunofluorescence analysis of Hela cells using anti-MLH1 mAb (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.| Product Name | MLH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FCC2; COCA2; HNPCC |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4C9C7 |
| Host Species | Mouse |
| Tested Applications | WBIHCIF |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | COCA2 |
|---|---|
| Alternative Names | FCC2 COCA2 HNPCC |
| Molecular Weight(MW) | 84601 Da |
| Function | Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS- heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis |
| Tissue Specificity | Colon, lymphocytes, breast, lung, spleen, testis, prostate, thyroid, gall bladder and heart |
| Cellular Localization | Nucleus. |
| Entrez Gene | 4292 |
|---|---|
| Nucleotide Accession | NP_001161091.1 |
Application
Figure 1: Western blot analysis using anti-MLH1 mAb against Hela (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS (6) cell lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human rectum cancer (left) and ovarian cancer (right) tissues, showing nuclear localization with DAB staining using anti-MLH1 mAb.
Application
Figure 3: Confocal immunofluorescence analysis of Hela cells using anti-MLH1 mAb (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli. |
