Application
Western blot analysis of Neprilysin antibody (Center) in mouse kidney tissue lysates (35ug/lane). Neprilysin (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of MME Antibody (Center)(Cat#AP7329c) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Product Name | MME Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MME; EPN; Neprilysin; Atriopeptidase; Common acute lymphocytic leukemia antigen; Enkephalinase; Neutral endopeptidase 24.11; Skin fibroblast elastase; CD_antigen=CD10 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EPN |
|---|---|
| Alternative Names | MME EPN Neprilysin Atriopeptidase Common acute lymphocytic leukemia antigen Enkephalinase Neutral endopeptidase 24.11 Skin fibroblast elastase CD_antigen=CD10 |
| Molecular Weight(MW) | 85514 Da |
| Function | Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV- inducible elastase activity toward skin preelastic and elastic fibers |
| Tissue Specificity | This MME antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281~310 amino acids from the Center region of human MME. |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein |
| Entrez Gene | 4311 |
|---|
Application
Western blot analysis of Neprilysin antibody (Center) in mouse kidney tissue lysates (35ug/lane). Neprilysin (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of MME Antibody (Center)(Cat#AP7329c) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
