Application
Western blot analysis of Phospho-NFE2L2-Y576 in Jurkat cell line lysates (35ug/lane). NFE2L2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with NFE2L2-Y576 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
NFE2L2-Y576 Antibody flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | NFE2L2-Y576 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NRF2 |
|---|---|
| Alternative Names | NFE2L2 NRF2 Nuclear factor erythroid 2-related factor 2 HEBP1 Nuclear factor erythroid derived 2 like 2 |
| Molecular Weight(MW) | 67827 Da |
| Function | Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region |
| Tissue Specificity | Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle |
| Cellular Localization | Cytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents |
Application
Western blot analysis of Phospho-NFE2L2-Y576 in Jurkat cell line lysates (35ug/lane). NFE2L2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with NFE2L2-Y576 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
NFE2L2-Y576 Antibody flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
