Application
Western blot analysis of NKX3-1 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). NKX3-1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with NKX3-1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
NKX3-1 Antibody (Center) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | NKX3-1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NKX3.1, NKX3A |
|---|---|
| Alternative Names | NKX3-1 NKX3.1 NKX3A Homeobox protein Nkx-3.1 Homeobox protein NK-3 homolog A |
| Molecular Weight(MW) | 26350 Da |
| Function | Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells |
| Tissue Specificity | Highly expressed in the prostate and, at a lower level, in the testis |
| Cellular Localization | Nucleus. |
| Entrez Gene | 4824 |
|---|
Application
Western blot analysis of NKX3-1 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). NKX3-1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with NKX3-1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
NKX3-1 Antibody (Center) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
