Application
Figure 1: Western blot analysis using anti-P16 monoclonal antiobdy against truncated P16 recombinant protein.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded rat liver tissue(A), human brain tissue(B) and brain tumor(C), showing nuclear localization using anti-P16 antibody with DAB staining.| Product Name | P16 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P16 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone Number | 2D9A12 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDKN2 MLM |
|---|---|
| Alternative Names | P16 |
| Molecular Weight(MW) | 13903 Da |
| Function | Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus This inhibits the oncogenic action of MDM2 by blocking MDM2- induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with COMMD1 and promotes its 'Lys63'-linked polyubiquitination Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development. Isoform 6 may be involved in regulation of autophagy and caspase-independent cell death; the short-lived mitochondrial isoform is stabilized by C1QBP |
| Tissue Specificity | Purified recombinant fragment of P16 expressed in E. Coli. |
| Cellular Localization | Nucleus, nucleolus. Nucleus, nucleoplasm. |
Application
Figure 1: Western blot analysis using anti-P16 monoclonal antiobdy against truncated P16 recombinant protein.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded rat liver tissue(A), human brain tissue(B) and brain tumor(C), showing nuclear localization using anti-P16 antibody with DAB staining.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of P16 expressed in E. Coli. |
