Application
Figure 1: Western blot analysis using anti-P16 monoclonal antiobdy against truncated P16 recombinant protein.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded rat liver tissue(A), human brain tumor(B), breast cancer(C), esophageal epithelium tissue(D), mouse brain tissue(E) and stomach tisue(F), showing nuclear localization using anti-P16 antibody with DAB staining.| Product Name | P16 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P16 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1E12E10 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDKN2 MTS1 |
|---|---|
| Alternative Names | P16 |
| Molecular Weight(MW) | 16533 Da |
| Function | Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein |
| Tissue Specificity | Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific |
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Figure 1: Western blot analysis using anti-P16 monoclonal antiobdy against truncated P16 recombinant protein.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded rat liver tissue(A), human brain tumor(B), breast cancer(C), esophageal epithelium tissue(D), mouse brain tissue(E) and stomach tisue(F), showing nuclear localization using anti-P16 antibody with DAB staining.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of P16 expressed in E. Coli. |
