Application
Western blot analysis of anti-PAK6 Pab in lysates from transiently transfected COS7 cells. Lane 1: negative control, Lane 2: PAK1-expressing cells, Lane 3: PAK2-expressing cells, Lane 4: PAK4-expressing cells, Lane 5: PAK5-expressing cells, and Lane 6: PAK6-expressing cells. PAK6 (arrow) was detected using purified Pab. Data is kindly provided by Drs. Z.M. Jaffer and J. Chernoff from the Fox Chase Cancer Center (Philadelphia, PA).| Product Name | PAK6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PAK6; PAK5; Serine/threonine-protein kinase PAK 6; PAK-5; p21-activated kinase 6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PAK5 |
|---|---|
| Alternative Names | PAK6 PAK5 Serine/threonine-protein kinase PAK 6 PAK-5 p21-activated kinase 6 |
| Molecular Weight(MW) | 74869 Da |
| Function | Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Inhibits also ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD |
| Tissue Specificity | Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast |
| Cellular Localization | Cytoplasm. Nucleus. Note=Cotranslocates into nucleus with AR in response to androgen induction |
Application
Western blot analysis of anti-PAK6 Pab in lysates from transiently transfected COS7 cells. Lane 1: negative control, Lane 2: PAK1-expressing cells, Lane 3: PAK2-expressing cells, Lane 4: PAK4-expressing cells, Lane 5: PAK5-expressing cells, and Lane 6: PAK6-expressing cells. PAK6 (arrow) was detected using purified Pab. Data is kindly provided by Drs. Z.M. Jaffer and J. Chernoff from the Fox Chase Cancer Center (Philadelphia, PA).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
