Application
PAK6 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PAK6 antibody detected the PAK6 protein (arrow).Application
PAK6 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAK6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of PAK6 Antibody (Center)(Cat#AP12164c) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | PAK6 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PAK6; PAK5; Serine/threonine-protein kinase PAK 6; PAK-5; p21-activated kinase 6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PAK5 |
|---|---|
| Alternative Names | PAK6 PAK5 Serine/threonine-protein kinase PAK 6 PAK-5 p21-activated kinase 6 |
| Molecular Weight(MW) | 74869 Da |
| Function | Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Inhibits also ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD |
| Tissue Specificity | Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast |
| Cellular Localization | Cytoplasm. Nucleus. Note=Cotranslocates into nucleus with AR in response to androgen induction |
| Entrez Gene | 56924 |
|---|---|
| Nucleotide Accession | NP_001122100.1 |
Application
PAK6 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PAK6 antibody detected the PAK6 protein (arrow).
Application
PAK6 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAK6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of PAK6 Antibody (Center)(Cat#AP12164c) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
