Application
Dot blot analysis of anti-Phospho-RUNX2-pS465 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-RUNX2-pS465 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RUNX2; AML3; CBFA1; OSF2; PEBP2A; Runt-related transcription factor 2; Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1; Oncogene AML-3; Osteoblast-specific transcription factor 2; Polyomavirus enhancer-binding protein 2 alpha A subuni |
| Modification | p-S465 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:0.388888888889 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AML3 CBFA1 OSF2 PEBP2A |
|---|---|
| Alternative Names | RUNX2 AML3 CBFA1 OSF2 PEBP2A Runt-related transcription factor 2 Acute myeloid leukemia 3 protein Core-binding factor subunit alpha-1 Oncogene AML-3 Osteoblast-specific transcription factor 2 Polyomavirus enhancer-binding protein 2 alpha A subuni |
| Molecular Weight(MW) | 56648 Da |
| Function | Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'- PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation |
| Tissue Specificity | Specifically expressed in osteoblasts. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 860 |
|---|
Application
Dot blot analysis of anti-Phospho-RUNX2-pS465 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | DB:0.388888888889 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
