Application
RUNX2 Antibody (S533) western blot analysis in Hob cell line lysates.This demonstrates the RUNX2 antibody detected the RUNX2 protein (arrow)(Kindly provided by Dr. John Wu).Application
RUNX2 Antibody (S533) (AP7735d)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RUNX2 Antibody (S533) for immunohistochemistry. Clinical relevance has not been evaluated.Application
RUNX2 Antibody (S533) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | RUNX2 Antibody (S533) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RUNX2; AML3; CBFA1; OSF2; PEBP2A; Runt-related transcription factor 2; Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1; Oncogene AML-3; Osteoblast-specific transcription factor 2; Polyomavirus enhancer-binding protein 2 alpha A subuni |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AML3 CBFA1 OSF2 PEBP2A |
|---|---|
| Alternative Names | RUNX2 AML3 CBFA1 OSF2 PEBP2A Runt-related transcription factor 2 Acute myeloid leukemia 3 protein Core-binding factor subunit alpha-1 Oncogene AML-3 Osteoblast-specific transcription factor 2 Polyomavirus enhancer-binding protein 2 alpha A subuni |
| Molecular Weight(MW) | 56648 Da |
| Function | Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'- PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation |
| Tissue Specificity | Specifically expressed in osteoblasts. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 860 |
|---|
Application
RUNX2 Antibody (S533) western blot analysis in Hob cell line lysates.This demonstrates the RUNX2 antibody detected the RUNX2 protein (arrow)(Kindly provided by Dr. John Wu).
Application
RUNX2 Antibody (S533) (AP7735d)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RUNX2 Antibody (S533) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
RUNX2 Antibody (S533) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
