Application
Western blot analysis on (A) SH-SY-5Y, (B) U937, (C) Jurkat cell lysates using anti-PKN1 RabMAb (Cat. #AJ1626a).Application
Flow cytometric analysis of permeabilized Jurkat cells using anti- PKN1 RabMAb (red) (Cat. #AJ1626a) or a rabbit IgG (negative) (green).| Product Name | PKN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3238 |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:1000 ??10000 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PAK1 PKN PRK1 PRKCL1 |
|---|---|
| Molecular Weight(MW) | 103932 Da |
| Function | PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser- 159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro |
| Tissue Specificity | Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells |
| Cellular Localization | Cytoplasm. Nucleus. Endosome. Cell membrane; Peripheral membrane protein. Cleavage furrow. Midbody Note=Associates with chromatin in a ligand-dependent manner Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-374 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis |
| Entrez Gene | 5585 |
|---|
Application
Western blot analysis on (A) SH-SY-5Y, (B) U937, (C) Jurkat cell lysates using anti-PKN1 RabMAb (Cat. #AJ1626a).
Application
Flow cytometric analysis of permeabilized Jurkat cells using anti- PKN1 RabMAb (red) (Cat. #AJ1626a) or a rabbit IgG (negative) (green).| Application Notes | WB:1:1000 ??10000 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
