Application
A. Western blot analysis on K562 cell lysate using anti-PUMA (C-term) RabMAb (Cat. #AJ1666a), dilution 1:5,000.Application
B. Immunofluorescent staining of HeLa cells using anti-PUMA (C-term) RabMAb (Cat. #AJ1666a).Application
C. Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using anti-PUMA (C-term) RabMAb (Cat. #AJ1666a).Application
D. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-PUMA (C-term) RabMAb (Cat. #AJ1666a).| Product Name | PUMA Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP512Y |
| Host Species | Rabbit |
| Tested Applications | WBIFIHC |
| WB:1:1000~5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PUMA |
|---|---|
| Alternative Names | BBC3 PUMA Bcl-2-binding component 3 p53 up-regulated modulator of apoptosis JFY-1 |
| Molecular Weight(MW) | 20532 Da |
| Function | Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Regulates ER stress-induced neuronal apoptosis (By similarity) |
| Tissue Specificity | Ubiquitously expressed. |
| Cellular Localization | Mitochondrion. Note=Localized to the mitochondria in order to induce cytochrome c release |
| Entrez Gene | 27113 |
|---|
Application
A. Western blot analysis on K562 cell lysate using anti-PUMA (C-term) RabMAb (Cat. #AJ1666a), dilution 1:5,000.
Application
B. Immunofluorescent staining of HeLa cells using anti-PUMA (C-term) RabMAb (Cat. #AJ1666a).
Application
C. Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using anti-PUMA (C-term) RabMAb (Cat. #AJ1666a).
Application
D. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-PUMA (C-term) RabMAb (Cat. #AJ1666a).| Application Notes | WB:1:1000~5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
