Application
Western blot analysis of anti-Puma BH3 domain Pab in HL-60 cell lysate. Puma BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Puma BH3 Domain antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of Puma BH3 Domain Antibody(Cat#AP1317a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
Puma BH3 Domain Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | Puma BH3 Domain Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PUMA |
|---|---|
| Alternative Names | BBC3 PUMA Bcl-2-binding component 3 JFY-1 p53 up-regulated modulator of apoptosis |
| Molecular Weight(MW) | 20532 Da |
| Function | Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Regulates ER stress-induced neuronal apoptosis (By similarity) |
| Tissue Specificity | Ubiquitously expressed. |
| Cellular Localization | Mitochondrion. Note=Localized to the mitochondria in order to induce cytochrome c release |
| Entrez Gene | 27113 |
|---|
Application
Western blot analysis of anti-Puma BH3 domain Pab in HL-60 cell lysate. Puma BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Puma BH3 Domain antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of Puma BH3 Domain Antibody(Cat#AP1317a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
Puma BH3 Domain Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
