Application
Figure 1: Western blot analysis using anti-Rab25 monoclonal antiobdy against truncated Rab25 recombinant protein(1), human overy carcinoma(2), stomach carcinoma(3), breast carcinoma(4) tissue lysate.Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human bladder carcinoma(A), breast carcinoma(B), esophagus carcinoma(C), skin carcinoma(D) tissue, showing cytoplasmic localization using anti-Rab25 antibody with DAB staining.| Product Name | Rab25 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CATX-8; RAB25 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 3F12F3 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CATX8 |
|---|---|
| Alternative Names | CATX-8 RAB25 |
| Molecular Weight(MW) | 23496 Da |
| Function | Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia. Increases the rates of ovarian and breast cancers progression and aggressiveness by modulating cellular processes such as proliferation, survival and migration of epithelial tumor cells. May selectively regulate the apical recycling pathway Together with MYO5B regulates transcytosis (By similarity) |
| Tissue Specificity | Expressed in ovarian epithelium (NOE) and breast tissue. Expressed in ovarian cancer; expression is increased relative to NOE cells. Expression in ovarian cancer is stage dependent, with stage III and stage IV showing higher levels than early stage cancers. Expressed in breast cancer; expression is increased relative to normal breast tissue |
| Cellular Localization | Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Cell projection, pseudopodium membrane Cytoplasmic vesicle. Note=Colocalizes with integrin alpha-V/beta-1 in vesicles at the pseudopodial tips |
| Entrez Gene | 57111 |
|---|---|
| Nucleotide Accession | NP_065120.2 |
Application
Figure 1: Western blot analysis using anti-Rab25 monoclonal antiobdy against truncated Rab25 recombinant protein(1), human overy carcinoma(2), stomach carcinoma(3), breast carcinoma(4) tissue lysate.
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human bladder carcinoma(A), breast carcinoma(B), esophagus carcinoma(C), skin carcinoma(D) tissue, showing cytoplasmic localization using anti-Rab25 antibody with DAB staining.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of Rab25 expressed in E. Coli. |
