Application
RAD17 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RAD17 antibody detected the RAD17 protein (arrow).Application
RAD17 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | RAD17 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAD17; R24L; Cell cycle checkpoint protein RAD17; RF-C/activator 1 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | R24L |
|---|---|
| Alternative Names | RAD17 R24L Cell cycle checkpoint protein RAD17 RF-C/activator 1 homolog |
| Molecular Weight(MW) | 77055 Da |
| Function | Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9- HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination |
| Tissue Specificity | Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors) |
| Cellular Localization | Nucleus. Note=Phosphorylated form redistributes to discrete nuclear foci upon DNA damage |
| Entrez Gene | 5884 |
|---|---|
| Nucleotide Accession | NP_579916.1 NP_002864.1 NP_579917.1 NP_579918.1 NP_579922.1 NP_579920.1 NP_579919.1 N |
Application
RAD17 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RAD17 antibody detected the RAD17 protein (arrow).
Application
RAD17 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
