Application
Western blot analysis of RAD17 antibody (N-term) in HL60 cell line lysates (35ug/lane). RAD17 (arrow) was detected using the purified Pab.Application
Flow cytometric analysis of hela cells using RAD17 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | RAD17 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAD17; R24L; Cell cycle checkpoint protein RAD17; RF-C/activator 1 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | R24L |
|---|---|
| Alternative Names | RAD17 R24L Cell cycle checkpoint protein RAD17 RF-C/activator 1 homolog |
| Molecular Weight(MW) | 77055 Da |
| Function | Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9- HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination |
| Tissue Specificity | Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors) |
| Cellular Localization | Nucleus. Note=Phosphorylated form redistributes to discrete nuclear foci upon DNA damage |
| Entrez Gene | 5884 |
|---|
Application
Western blot analysis of RAD17 antibody (N-term) in HL60 cell line lysates (35ug/lane). RAD17 (arrow) was detected using the purified Pab.
Application
Flow cytometric analysis of hela cells using RAD17 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
