Application
A. Western blot analysis on HeLa cell lysates using anti-Rad17 phospho (pS645) RabMAb (Cat. #AJ1671a), 1:5000 dilution. Cells were either (A) untreated (B) treated with Lambda Phosphatase.| Product Name | Rad17 Antibody Phospho (pS645) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAD17, R24L, Cell cycle checkpoint protein RAD17, RF-C/activator 1 homolog |
| Modification | p-S645 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1519Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500~5000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | R24L |
|---|---|
| Alternative Names | RAD17 R24L Cell cycle checkpoint protein RAD17 RF-C/activator 1 homolog |
| Molecular Weight(MW) | 77055 Da |
| Function | Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9- HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination |
| Tissue Specificity | Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors) |
| Cellular Localization | Nucleus. Note=Phosphorylated form redistributes to discrete nuclear foci upon DNA damage |
| Entrez Gene | 5884 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-Rad17 phospho (pS645) RabMAb (Cat. #AJ1671a), 1:5000 dilution. Cells were either (A) untreated (B) treated with Lambda Phosphatase.| Application Notes | WB:1:2500~5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
