Application
The anti-UBCE7IP3 Pab is used in Western blot to detect UBCE7IP3 in mouse kidney tissue lysate.Application
RBCK1 (UBCE7IP3) Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RBCK1 (UBCE7IP3) Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | RBCK1 (UBCE7IP3) Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RBCK1; C20orf18; RNF54; UBCE7IP3; XAP3; XAP4; RanBP-type and C3HC4-type zinc finger-containing protein 1; HBV-associated factor 4; Heme-oxidized IRP2 ubiquitin ligase 1; Hepatitis B virus X-associated protein 4; RING finger protein 54; Ubiquitin-conjugatin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C20orf18 RNF54 UBCE7IP3 XAP3 XAP4 |
|---|---|
| Alternative Names | RBCK1 C20orf18 RNF54 UBCE7IP3 XAP3 XAP4 RanBP-type and C3HC4-type zinc finger-containing protein 1 HBV-associated factor 4 Heme-oxidized IRP2 ubiquitin ligase 1 Hepatitis B virus X-associated protein 4 RING finger protein 54 Ubiquitin-conjugatin |
| Molecular Weight(MW) | 57572 Da |
| Function | E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteasome. Component of the LUBAC complex which conjugates linear ('M-1'-linked) polyubiquitin chains to substrates and plays a key role in NF- kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF- RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types |
| Tissue Specificity | This RBCK1 (UBCE7IP3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13~42 amino acids from the N-terminal region of human UBCE7IP3. |
| Entrez Gene | 10616 |
|---|
Application
The anti-UBCE7IP3 Pab is used in Western blot to detect UBCE7IP3 in mouse kidney tissue lysate.
Application
RBCK1 (UBCE7IP3) Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RBCK1 (UBCE7IP3) Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
Data sheet for OM219584