Application
RBCK1 Antibody (N-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the RBCK1 antibody detected the RBCK1 protein (arrow).| Product Name | RBCK1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RBCK1; C20orf18; RNF54; UBCE7IP3; XAP3; XAP4; RanBP-type and C3HC4-type zinc finger-containing protein 1; HBV-associated factor 4; Heme-oxidized IRP2 ubiquitin ligase 1; Hepatitis B virus X-associated protein 4; RING finger protein 54; Ubiquitin-conjugatin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C20orf18 RNF54 UBCE7IP3 XAP3 XAP4 |
|---|---|
| Alternative Names | RBCK1 C20orf18 RNF54 UBCE7IP3 XAP3 XAP4 RanBP-type and C3HC4-type zinc finger-containing protein 1 HBV-associated factor 4 Heme-oxidized IRP2 ubiquitin ligase 1 Hepatitis B virus X-associated protein 4 RING finger protein 54 Ubiquitin-conjugatin |
| Molecular Weight(MW) | 57572 Da |
| Function | E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteasome. Component of the LUBAC complex which conjugates linear ('M-1'-linked) polyubiquitin chains to substrates and plays a key role in NF- kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF- RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types |
| Tissue Specificity | This RBCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RBCK1. |
| Entrez Gene | 10616 |
|---|---|
| Nucleotide Accession | NP_112506.2 |
Application
RBCK1 Antibody (N-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the RBCK1 antibody detected the RBCK1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Data sheet for OM219587