Application
A. Western blot analysis on Jurkat cell lysate using anti-Smac/Diablo RabMAb (Cat. #AJ1721a), 1:1,000 dilution.Application
B. Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using anti-Smac/Diablo RabMAb (Cat. #AJ1721a).| Product Name | Smac/Diablo Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DIABLO, SMAC, Diablo homolog, mitochondrial, Direct IAP-binding protein with low pI |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y12 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SMAC |
|---|---|
| Alternative Names | DIABLO SMAC Diablo homolog mitochondrial Direct IAP-binding protein with low pI |
| Molecular Weight(MW) | 27131 Da |
| Function | Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP) Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance |
| Tissue Specificity | Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed |
| Cellular Localization | Mitochondrion. Note=Released into the cytosol when cells undergo apoptosis |
| Entrez Gene | 56616 |
|---|
Application
A. Western blot analysis on Jurkat cell lysate using anti-Smac/Diablo RabMAb (Cat. #AJ1721a), 1:1,000 dilution.
Application
B. Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using anti-Smac/Diablo RabMAb (Cat. #AJ1721a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
