Application
IHC analysis of human prostate using Smac antibody at 10 µg/ml.Application
Western blot analysis of Smac (DIABLO) in HeLa lysate using Smac antibody at 2 ug/ml.| Product Name | Diablo antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Smac / DiabloInhibitors of apoptosis proteins (IAP's) block cell death by caspase inhibition. X linked inhibitor of apoptosis (XIAP) is a member of the IAP family. Smac/Diablo is expressed in a variety of tissues and has been identified as a negative regulator of XIAP. Smac/Diablo is a mitochondrial protein that enters the cytosol when cells undergo apoptosis, and moderates the caspase inhibition of IAPs. |
| Immunogen | Two synthetic peptides corresponding to amino acids 199 to 212 and amino acids 226 to 239 of human Smac/DIABLO |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 - 2 mg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27.1 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | SMAC Q9NR28 56616 FLJ10537 605219 DIABLO-S Diablo DIABLOS SMAC3 FLJ25049 DIABLO S |
|---|---|
| Target | Diablo |
| Cellular Localization | Mitochondrial but released into the cytosol when cells undergo apoptosis. |
Application
IHC analysis of human prostate using Smac antibody at 10 µg/ml.
Application
Western blot analysis of Smac (DIABLO) in HeLa lysate using Smac antibody at 2 ug/ml.| Positive Control | HeLa cell lysate |
|---|---|
| Application Notes | IHC: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 - 2 mg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27.1 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
