Application
Western Blot: Smac Antibody (78-1-118) [ - HeLa whole cell extract lysates.| Product Name | Diablo antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal to Smac / DiabloSmac/DIABLO, a mitochondrial protein, activates various forms of apoptosis. This activation may be due to the neutralization of one or more members of the IAP family of apoptosis inhibitory proteins. Smac exits the mitochondria and enter the cytosol during certain apoptosis triggered events. Mitochondrial-mediated apoptosis is important in animal development and tissue homeostasis, with alterations resulting in a range of malignant disorders. Upon apoptotic stimuli, the mitochondrial proteins cytochrome c and Smac/DIABLO are released into the cytosol. The release of these proteins, however, occurs via different mechanisms (2,3). DIABLO-S antibody, FLJ10537 antibody, FLJ25049 antibody, SMAC antibody, SMAC3 antibody |
| Immunogen | Recombinant mature human Smac/DIABLO (lacks amino acids 1-55 present in Smac/DIABLO precursor) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 78-1-118 |
| Host Species | Mouse |
| Tested Applications | WB |
| For WB: Use at a dilution of 1:500 (ECL). Works in western analysis where a band is seen at ~25 kDa representing Smac/DIABLO. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SMAC Q9NR28 56616 FLJ10537 605219 DIABLO-S Diablo DIABLOS SMAC3 FLJ25049 DIABLO S |
|---|---|
| Target | Diablo |
| Tissue Specificity | This antibody is specific for Smac/DIABLO. In Western blots of 293 cells, this antibody shows a single band. |
Application
Western Blot: Smac Antibody (78-1-118) [ - HeLa whole cell extract lysates.| Positive Control | 293 cell extract. |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500 (ECL). Works in western analysis where a band is seen at ~25 kDa representing Smac/DIABLO. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Ascitic fluid containing 0.1% sodium azide |
