Application
Western blot analysis of ST8SIA4 Antibody (Center) in HL-60 cell line lysates (35ug/lane). ST8SIA4 (arrow) was detected using the purified Pab. Western blot analysis of ST8SIA4 Antibody (Center) in mouse spleen tissue lysates (35ug/lane). ST8SIA4 (arrow) was detected using the purified Pab.Application
ST8SIA4 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ST8SIA4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
ST8SIA4 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ST8SIA4 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ST8SIA4; PST; PST1; SIAT8D; CMP-N-acetylneuraminate-poly-alpha-2, 8-sialyltransferase; Alpha-2, 8-sialyltransferase 8D; Polysialyltransferase-1; Sialyltransferase 8D; Sialytransferase St8Sia IV |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PST PST1 SIAT8D |
|---|---|
| Alternative Names | ST8SIA4 PST PST1 SIAT8D CMP-N-acetylneuraminate-poly-alpha-2 8-sialyltransferase Alpha-2 8-sialyltransferase 8D Polysialyltransferase-1 Sialyltransferase 8D Sialytransferase St8Sia IV |
| Molecular Weight(MW) | 41295 Da |
| Function | Catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid (PSA), which is present on the embryonic neural cell adhesion molecule (N-CAM), necessary for plasticity of neural cells |
| Tissue Specificity | Highly expressed in fetal brain, lung and kidney and in adult heart, spleen and thymus. Present to a lesser extent in adult brain, placenta, lung, large and small intestine and peripheral blood leukocytes |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein (Potential) |
| Entrez Gene | 7903 |
|---|
Application
Western blot analysis of ST8SIA4 Antibody (Center) in HL-60 cell line lysates (35ug/lane). ST8SIA4 (arrow) was detected using the purified Pab. Western blot analysis of ST8SIA4 Antibody (Center) in mouse spleen tissue lysates (35ug/lane). ST8SIA4 (arrow) was detected using the purified Pab.
Application
ST8SIA4 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ST8SIA4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
ST8SIA4 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
